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اکتبر 20, 2017
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اکتبر 20, 2017
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Pulsed Field Gel Electrophoresis Systems

 

CHEF Mapper® XA System

The CHEF Mapper® XA system incorporates patented FIGE and AFIGE* technologies for superior resolution in the range of 100 bp to 10 Mb, in addition to the features of the CHEF-DR® II and the CHEF-DR III systems. The CHEF Mapper XA system also includes algorithms for deriving separation conditions. This system is ideal for both the PFGE novice and the seasoned expert.
Each CHEF Mapper XA system is supplied with a power module, embedded auto-algorithm for protocol optimization, interactive algorithm program disk, electrophoresis cell, cooling module, variable-speed pump, and accessory kit.
Specifications :

Automation
The CHEF Mapper XA system offers two ways to optimize your separations. The unique auto-algorithm can automatically select optimal separation conditions, integrating 11 key variables and implementing starting separation conditions. Protocols can be refined using the Windows operating system-based interactive algorithm, which lets you specify several run variables simultaneously to derive optimal separation protocols.
Customization
Busy laboratories need equipment that can store and readily access key separation conditions. The CHEF Mapper XA system can store up to 99 programs.
Application Versatility
The CHEF Mapper XA system is the most flexible of the PFGE units. The CHEF Mapper XA system achieves higher resolution with greater speed and accuracy than any other PFGE system, making it ideal for all PFGE applications. The CHEF Mapper XA system lets you choose any pulse angle from 0 to 360°, which allows optimal separation of both chromosomal and plasmid DNA with one system. Accurate sizing of fragments requires an expanded linear range of separation. Switch-time ramps increase the mobility of fragments by gradually changing the switch times during the course of a run. Nonlinear (for example, concave or convex) ramps change the switch-time increments from the start to the end of a run. These nonlinear ramps separate fragments linearly from 50 to 700 kb, yielding more precise fragment size measurements. The multistate mode of the CHEF Mapper XA system enhances resolution in selected fragment size ranges. Each vector (angle of pulse) can be assigned its own voltage (field intensity) and its own switch time (duration of pulse). Up to eight different states can be combined into one run to optimize the separation of subsets of fragments in the sample. The application of secondary pulses of defined voltage, duration, angle, and frequency can enhance the separation and resolution of very large DNA molecules by releasing DNA caught in the gel matrix.
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CHEF-DR® II

CHEF technology yields straight lanes over a wide range of DNA molecule sizes. The CHEF-DR® II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.
The CHEF-DR II system effectively resolves DNA fragments in the range of 5 kb to 6 Mb. This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system exclusively employs the most common angle of electrophoresis for PFGE, 120°. This system is simple to program, and has proven to be a solid performer. Each CHEF-DR II system is supplied with an electrophoresis cell, drive module, control module, variable-speed pump, and accessory kit. The CHEF-DR II chiller systems also come with a cooling module.
Specifications :

Automation
The CHEF-DR II system, the most cost-effective PFGE instrument, requires direct user input of optimal run time, voltage gradient, and switch intervals for each run.
Customization
Users can program run conditions into the CHEF-DR II system. The product manual features extensive examples of run conditions that can be input based on a variety of size separation ranges.
Application Versatility
The CHEF-DR II system is optimized for the most popular range of separations with a fixed angle of 120°. This unit can be used for separations of fragments up to 6 Mb by adjusting the run conditions for low voltage and extended run times.
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CHEF-DR III

The CHEF-DR® III variable angle system combines patented PACE technology and proven CHEF technology into one easy-to-use instrument that lets you use up to three blocks of run conditions to generate high-resolution separations; specify any electrophoresis angle from 90 to 120°. Separation of chromosome-sized DNA can be achieved in almost half the time previously possible, without sacrificing resolution.
Each CHEF-DR III system is supplied with a power module, electrophoresis cell, variable-speed pump, and accessory kit.The CHEF-DR III chiller systems also come with a cooling module.
Specifications :

Automation
The CHEF-DR III system has built-in automation. It will recall the last conditions and use them as the default protocol. The CHEF-DR III system will also recall current run conditions and run progress if interrupted by power failure and resume the run without user intervention.
Customization
With the CHEF-DR III system, conditions are entered by the user. The instruction manual describes extensive examples of run conditions based on a variety of size separation ranges.
Application Versatility
The ability to program the angle of electrophoresis with the CHEF-DR III system enables fast, high-resolution separations of DNA molecules ranging from 200 kb to >6 Mb. The CHEF-DR III system allows selection of the optimal voltage gradient, switch time, and angle for the specific DNA size range. Separations can be further optimized by programming up to three consecutively executing blocks of run conditions, increasing the variety of possible run conditions.
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