Electroporation
اکتبر 17, 2017
Gel Documentation systems
اکتبر 20, 2017
Biolistic particle Delivery Systems
Helios® gene gun and PDS-1000/He™ systems use a helium pulse to accelerate gold or tungsten particles coated with DNA directly into target cells, including cultures, tissues, plants, and more.
Helios Gene Gun System
The Helios gene gun is a convenient handheld device that provides rapid and direct transfection into a range of targets in vivo. The unit uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers from the inner wall of a small plastic cartridge directly into target cells.
Description :
Key Features
• Provides easy-to-use, rapid, versatile gene delivery independent of target cell type
• Facilitates both transient and stable expression
• Requires only small amounts of DNA and cells; no carrier DNA needed
• Enables codelivery of more than one plasmid
• Allows transfer of large DNA fragments
• Targets intracellular gene delivery to many cells
• Works for both in vitro and in vivo transformation
• Delivers no extraneous genes or proteins
دریافت کاتالوگ
Description :
Key Features
• Provides easy-to-use, rapid, versatile gene delivery independent of target cell type
• Facilitates both transient and stable expression
• Requires only small amounts of DNA and cells; no carrier DNA needed
• Enables codelivery of more than one plasmid
• Allows transfer of large DNA fragments
• Targets intracellular gene delivery to many cells
• Works for both in vitro and in vivo transformation
• Delivers no extraneous genes or proteins
دریافت کاتالوگ
PDS-1000/He™ and Hepta Systems
The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.
The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells
Specifications :
Key Features
• A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
• Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
• Maximum control
• High reproducibility
PDS-1000/He System with Hepta™ Adaptor
The Hepta adaptor for the PDS-1000/He biolistic system enables 7–10 times more cells to be transformed than the standard system. It fits into the shocking chamber of the PDS-1000/He system where it splits the helium shock wave over seven macrocarriers. By uniformly spreading the DNA-coated gold or tungsten particles over this larger area, the system maximizes the number of cells transformed during one bombardment. As the helium is split seven ways, pressure and particle velocities are reduced, making the system ideal for plants and cell cultures requiring less forceful penetration.
دریافت کاتالوگ
The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells
Specifications :
Key Features
• A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
• Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
• Maximum control
• High reproducibility
PDS-1000/He System with Hepta™ Adaptor
The Hepta adaptor for the PDS-1000/He biolistic system enables 7–10 times more cells to be transformed than the standard system. It fits into the shocking chamber of the PDS-1000/He system where it splits the helium shock wave over seven macrocarriers. By uniformly spreading the DNA-coated gold or tungsten particles over this larger area, the system maximizes the number of cells transformed during one bombardment. As the helium is split seven ways, pressure and particle velocities are reduced, making the system ideal for plants and cell cultures requiring less forceful penetration.
دریافت کاتالوگ
