Electroporation

The Gene Pulser Xcell™ system is a modular electroporation system for transfecting every cell type. The system includes a main unit, a ShockPod™ cuvette chamber, and your choice of accessory modules: the capacitance extender (CE module) and the pulse controller (PC module)
Specifications :

Gene Pulser Xcell™ Total System.  Includes : main unit, CE module, PC module, and ShockPod™ cuvette chamber
Waveform: Exponential or square Voltage: 10–3,000 V	Outputs
10–500 V, 25–3,275 µF in 25 µF increments 500–3,000 V, 10, 25, 50 µF	Capacitance
50–1,000 Ω in 50 Ω increments, plus infinity	Resistance (parallel)
20 Ω minimum at 10–2,500 V 600 Ω minimum at 2,500–3,000 V	Sample resistance
10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval 500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval	Square-wave timing
Gene Pulser Xcell Eukaryotic System. Includes :  main unit, CE module, and ShockPod cuvette chamber
Same as total system without the parallel resistance	Outputs and other specifications
Gene Pulser Xcell Microbial System. Includes : main unit, PC module, and ShockPod cuvette chamber
Waveform: Exponential or square Voltage: 200–3,000 V	Outputs
10, 25, 50 µF	Capacitance
50–1,000 Ω in 50 Ω increments, plus infinity	Resistance (parallel)
20 Ω minimum at 200–2,500 V 600 Ω minimum at 2,500–3,000 V	Sample resistance
0.05–5 ms pulse duration in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval	Square-wave timing
Gene Pulser Xcell Main Unit
Waveform: Exponential or square Voltage: 200–3,000 V	Outputs
10, 25, 50 µF	Capacitance
20 Ω minimum at 200–2,500 V 600 Ω minimum at 2,500–3,000 V	Sample resistance
0.05–5 ms pulse duration in 0.05 ms increments, 1–2 pulses, 5	Square-wave timing

Features and Benefits
• Universal electroporation — transfects all cell types, from primary and stem cells to bacteria and yeast
• Preset protocols — include the most common mammalian and bacterial cell types
• Flexibility — choice of programs for preset protocols, optimization protocols, manual operation, and/or user protocols
• Protocol library — collection of electroporation protocols for every cell type including primary, immortal, and bacterial cells
• Data management — enables storage and recall of parameters used in the previous 100 experiments, useful for troubleshooting
• Reproducibility — uses PulseTrac™ circuitry and arc-protection features to ensure reproducibility and sample protection
System Options
• Gene Pulser Xcell total electroporation system (catalog #165-2660) — the complete electroporation system for transfection of both eukaryotic and prokaryotic cells; includes both the CE module, and the PC module
• Gene Pulser Xcell eukaryotic electroporation system (catalog #165-2661) — for the electroporation of most eukaryotic cells, including mammalian cells and plant protoplasts; includes the CE module
• Gene Pulser Xcell microbial electroporation system (catalog #165-2662) — for the electroporation of bacteria and fungi, as well as other applications where high-voltage pulses are applied to samples of small volume; includes the PC module
More Information
• Features modifiable delivery parameters including time constant, actual voltage applied, pulse interval, and pulse time, depending on the waveform (exponential or square) chosen
• Provides a choice of programs for manual operation, preset protocols, user protocols, and an optimization protocol
• Compatible with 0.1 cm, 0.2 cm, and 0.4 cm Gene Pulser®/Micropulser™ electroporation cuvettes
• Compatible with any electroporation buffer, including Bio-Rad’s Gene Pulser electroporation buffer
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The MicroPulser electroporator is a simple yet versatile instrument that enables safe and reproducible transformation of bacteria, yeast, and other microorganisms. Transformation efficiencies much higher than those obtained with chemical methods can be achieved.
Specifications :

Key Features
• • One-button pulse delivery, attached cuvette chamber, and rapid charge time for fast sample handling
• Preset, optimized programs for commonly studied bacteria and fungi allow rapid program selection
• Arc quenching (ARQ) system significantly reduces arcing, protecting against loss of valuable samples
• Broad range of parameters for manual optimization. Manual programming allows voltage to be selected in a 200–3,000 V range with 10 V precision and allows pulse width to be selected in a 1.0–4.0 ms range with 0.1 ms precision
• High-voltage 3,000 V capability yields improved efficiency in larger-volume cuvettes
• Compact, space-saving design
• Audible and visible pulse indicators
• Time constant and actual volts displayed to monitor reproducibility.
دریافت کاتالوگ